To determine the role of dietary BCAA ingestion and omittance on MPS and insulin sensitivity
ID
Source
Brief title
Condition
- Glucose metabolism disorders (incl diabetes mellitus)
- Glucose metabolism disorders (incl diabetes mellitus)
- Muscle disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Muscle protein synthesis rates, expressed as fractional synthetic rate (FSR),
in the basal and postprandial state.
Secondary outcome
Muscle glucose uptake (i.e. direct measure of muscle insulin sensitivity).
Fat and fat-free mass (via DXA scan)
Background summary
The loss of muscle mass with ageing is accompanied by reduced physical
performance, loss of functional capacity, increased risk of falling and
increased likelihood of developing chronic metabolic diseases. The building
blocks of our muscles, called amino acids, are important for the quantity and
health of our muscles. Branched-chain amino acids (BCAAs, i.e. leucine,
isoleucine, and valine) are a specific subgroup of amino acids thought to be
important for maintaining muscle mass, but may also prevent muscles from
getting important nutrients (i.e. glucose) from the blood. Effect of BCAA on
muscle protein synthesis (MPS) predominantly done by supplementing. However,
the effect of acute ingestion of BCAA on MPS and glucose uptake (i.e. direct
measure of muscle insulin sensitivity) is currently not known.
Study objective
To determine the role of dietary BCAA ingestion and omittance on MPS and
insulin sensitivity
Study design
Randomized, parallel (two groups) study design.
Intervention
A single test day during which participants will orally ingest a dietary
supplement containing all amino acids (FullAA) or a supplement containing all
amino acids but BCAAs (NoBCAA). Continuous intravenous tracer infusion of
labelled amino acids will be combined with repeated blood and muscle samples.
Study burden and risks
The risks associated with this research are minimal. The muscle biopsies will
be taken through a small (5 mm) incision, after local anesthesia of the skin
and muscle fascia. These incisions will heal completely. Three intravenous
cannulas will be placed, which could lead to a local hematoma. Insertion of the
cannulas may cause pain, swelling, bruising, dizziness, fainting and very
rarely, clot formation, nerve damage and/or infection at the needle stick site.
There are no risks associated with the intravenous infusion of amino acids and
the oral intake of commercially available amino acids. When making the DXA
scan, a very low amount of X-ray radiation is used. The total exposure to
radiation as a result of the DXA scan is so low that it poses no health risks.
De Elst 1
Wageningen 6708 WD
NL
De Elst 1
Wageningen 6708 WD
NL
Listed location countries
Age
Inclusion criteria
• Aged from 18-35 years
• 18.5 < BMI < 30 kg·m2
• Recreationally active (performing non-competitive physical exercise at least
one time per week for minimally 30 minutes)
Exclusion criteria
• Smoking
• Diabetes (Type 1, Type 2, or genetic form of diabetes)
• Any diagnosed cardiovascular (heart) disease or high blood pressure (>=140
mmHg systolic and/or >=90 mmHg diastolic)
• Chronic use of any prescribed or over the counter pharmaceuticals (excluding
oral contraceptives and contraceptive devices)
• Known allergy to lidocaine
• Prone to keloid forming (i.e. hyperplastic growth of scars).
• Regular use of dietary protein and/or amino acid supplements (>3 times per
week)
• Currently involved in a structured progressive resistance training programme
(>3 times per week)
• A personal or family history of thrombosis (clots), epilepsy, seizures, or
schizophrenia.
• Any previous motor disorders or disorders in muscle and/or lipid metabolism
• Presence of an ulcer in the stomach or gut and/or strong history of
indigestion
• Known severe kidney problems
• Pregnant or breastfeeding
• Unable to give consent
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL82984.028.23 |