To provide evidence for the efficacy of Branched Chain Keto Acids (BCKA) and Branched Chain Amino Acids (BCAA) when compared with milk proteins to stimulate postprandial muscle protein synthesis in vivo in healthy older humans.
ID
Source
Brief title
Condition
- Other condition
- Muscle disorders
Synonym
Health condition
Het onderzoek zal worden uitgevoerd in gezonde proefpersonen
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Primary study parameters include post-prandial plasma amino acid availability
and myofibrillar muscle protein synthesis rates.
Secondary outcome
Secondary study parameters include whole-body protein synthesis, breakdown,
oxidation, and net balance.
Background summary
Protein and/or essential amino acids are important for stimulating muscle
protein synthesis (MPS) rates and inhibiting muscle protein breakdown. The
anabolic properties to protein feeding can be increased by the co-ingestion of
free leucine, thereby increasing the amount of dietary protein derived amino
acids that are used for de novo muscle protein synthesis. Consequently branched
chain amino acid (BCAA) supplementation or fortification can be used to
increase MPS. However, supplementing with high amounts of protein and/or BCAA,
necessary to stimulate MPS, can be harmful for patients suffering from for
example Chronic Kidney Disease (CKD). Supplementation with branched chain keto
acids (BCKA) may be of particular relevance in these conditions as these keto
analogues do not provide nitrogen (N) and may help to reduce metabolic workload
of liver and kidneys. However, it remains to be established whether BCAA and
BCKA can be useful in stimulating MPS in vivo in humans.
Study objective
To provide evidence for the efficacy of Branched Chain Keto Acids (BCKA) and
Branched Chain Amino Acids (BCAA) when compared with milk proteins to stimulate
postprandial muscle protein synthesis in vivo in healthy older humans.
Study design
Randomized double-blind, parallel design study.
Intervention
A beverage (300 mL) containing 6 g of BCAA, 6 g of BCKA- or 30 g of milk
protein will be consumed (n=15 per group).
Study burden and risks
The burden and risks associated with participation are small. Insertion of the
catheters is comparable to a blood draw and could result in a small hematoma.
Muscle biopsies will be taken under local anesthesia by an experienced
physician, but may cause some minor discomfort for maximally up to 24 h after
completion. The discomfort is comparable to muscle soreness or the pain one has
after bumping into a table. We will take 5 and 17 blood samples (10 mL) during
the screening and experimental trial respectively. The total amount of blood we
draw is less than half the amount of a blood donation and will be completely
restored in approximately 1 month. Participants come to the university twice: 1
screening (4 hours) and 1 experimental trial (entire day). For both the
screening and the experimental trial, participants have to be fasted, so they
are not allowed to eat and drink (except for water) from 22h00 the evening
before. Also, 3 days prior to the experimental trial participants should keep
their diet as constant as possible, do not perform any type of intense physical
exercise, and do not consume alcohol. During the screening we will perform a
DEXA and an OGTT. Furthermore, we will ask the participants to fill out a
medical questionnaire and record their food intake and activity for 2 days
prior to the experimental trial. During the experimental trial, we will collect
muscle and blood samples, and participants have to consume a beverage
containing protein (Milk), amino acids (BCAA) or keto acids (BCKA). There is no
direct benefit for the participants, only their contribution to scientific
knowledge and nutritional strategies that prevent muscle loss in the elderly,
which will be obtained from this study and used in the future.
Rellinghauser Straße 1-11
Essen 45128
DE
Rellinghauser Straße 1-11
Essen 45128
DE
Listed location countries
Age
Inclusion criteria
Healthy males
Age between 65 and 80 y
BMI between 18.5 and 30 kg/m2
Exclusion criteria
Lactose intolerance
Smoking
Diabetes
Diagnosed GI tract diseases
Arthritic conditions
A history of neuromuscular problems
Any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
Use of anticoagulants
Participation in exercise program
Hypertension, high blood pressure that is above 140/90 mmHg
Females (in order to have a more homogenous group)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL58952.068.16 |