The primary objective of this study is to assess the effect of a 16-day low protein diet on reward response when exposed to sight and smell of food stimuli high and low in protein compared with a high protein diet.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
obesitas
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Our main outcome measurement is the change in brain reward response to sight
and smell of high and low protein food stimuli after the 16-day low protein and
high protein diets.
Secondary outcome
• To assess the effect of a 16-day low protein diet on different aspects of
food reward: the explicit liking, the explicit wanting, the implicit wanting
and a forced food choice of high and low protein food using the computerized
Leeds Food Preference Questionnaire (LFPQ) compared with a high protein diet.
• To assess the effect of a 16-day low protein diet on protein and energy
intake compared with a high protein diet.
Background summary
Protein is an indispensable component within the human diet. It has been posed
that protein intake is tightly regulated in the human body. In a previous study
we showed that following a protein deficit, food intake and food preferences
changed to restore adequate protein status.
Study objective
The primary objective of this study is to assess the effect of a 16-day low
protein diet on reward response when exposed to sight and smell of food stimuli
high and low in protein compared with a high protein diet.
Study design
The study will consist of a 16-day fully controlled dietary intervention that
will involve consumption of individualized, isoenergetic menus providing either
0.5 g protein/kg BW/day (low protein diet), or 2.0 g protein/kg BW/day (high
protein diet), using a randomized crossover design. The diets will be followed
by a 1-day ad libitum-phase, where protein (g) and energy (kJ) intake will be
measured. Changes of reward responses in the brain will be measured by using
functional magnetic resonance imaging (fMRI) when exposed to sight and smell of
high and low protein food stimuli. Food preference will be measured by
subjective ratings.
Intervention
The study will consist of a 16-day fully controlled dietary intervention that
will involve consumption of individualized, isoenergetic menus providing either
0.5 g protein/kg BW/day (low protein diet), or 2.0 g protein/kg BW/day (high
protein diet).
Study burden and risks
The study duration will consist of 2 periods where subjects will follow a
controlled diet (two times 16 days). Afterwards there will be 1 ad libitum day
where subjects can eat as much as they want of the products we offer. After the
two diets participants will visit the fMRI facility in Ede (Hospital Gelderse
Vallei) to undergo fMRI measurements (in total 3 times, including 1 baseline
measurement). The study is non-therapeutic to the participants. The risk
associated with participation is negligible.
P.O. Box 8129
6700 EV Wageningen
NL
P.O. Box 8129
6700 EV Wageningen
NL
Listed location countries
Age
Inclusion criteria
Pag. 8 protocol:
• Age: 18-35 years
• BMI: 18.5 - 25.0 kg/m2
• Healthy (as judged by the participant)
Exclusion criteria
Pag. 8 protocol:
• Restraint eating (men: score > 2.25; women: score > 2.80)
• Lack of appetite
• Having difficulties with swallowing/eating
• Usage of an energy restricted diet during the last two months
• Weight loss or weight gain of 5 kg or more during the last two months
• Stomach or bowel diseases
• Kidney disorders
• Diabetes, thyroid disease, other endocrine disorders
• Having a history of neurological disorders
• Having taste or smell disorders
• Usage of daily medication other than birth control pills
• Pregnant or lactating
• Smoking more than one cigarette a day
• Being a vegetarian
• Being allergic/intolerant for products under study
• Working at the division of human nutrition (WUR)
• Having participated in *ProTime*, or current participation in other research from the division of
Human Nutrition (WUR).
• Having a contra-indication to MRI scanning (see page 9 of protocol)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL39292.081.11 |