Characterization of the effects of ageing on the innate immune response against influenza.
ID
Source
Brief title
Condition
- Viral infectious disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
To determine functional immune responses, after in vitro influenza infection of
dendritic cells isolated from young adults and elderly people. The responses
will be characterized based on; influenza-specific mRNA expressiom, maturation
molecule expression, inhibitory molecule expression, cytokine production and T
cell stimulating capacity.
Secondary outcome
To determine the variation of the assays for qualifying the innate immune
response of DC isolated from young adults or elderly individuals.
Background summary
Influenza cause high morbifdity and mortality rates in the elderly population
(> 65 years). There are indications that the function of the immune system
decreases with increasing age, and that this results in a decreased protection
against influenza infections in the elderly. For the induction of protective
immune responses after vaccination against influenza, the innate immune
response as provided by dendritic cells (DC) is of great importance. The DC
steer the influenza specific T cells by presenting the viral antigens. It is
therefore necessary to gain more insight into the effect of ageing on DC
function. The gained knowledge of this study will support the development of
new and/or improved vaccines against influenza for specifically the elderly
population.
Study objective
Characterization of the effects of ageing on the innate immune response against
influenza.
Study design
This study has been designed as an observational study. Subjects from two
groups, i.e. healthy young adults and healthy elderly, will be recruited by the
Julius Center at the UMC Utrecht or RIVM/NVI. Each subject will be screened for
the in- and exclusion criteria. From each participant, 60 ml of blood will be
drawn once for isolation of peripheral blood mononuclear cells (PBMC), which
will be used for the culturing of monocyte-derived DC. The DC will be infected
in vitro with influenza and subsequently tested for phenotypic and functional
immune response. Responses from young and elderly will be compared.
Study burden and risks
Possibly hematoma, pain from blood drawing. Participation in this study will
support the development of improved vaccines against influenza for the elderly.
Antonie van Leeuwenhoeklaan 11
3720 AL Bilthoven
Nederland
Antonie van Leeuwenhoeklaan 11
3720 AL Bilthoven
Nederland
Listed location countries
Age
Inclusion criteria
Subjects have to fulfil all the following criteria:
- 20-40 years or >= 65 years old
- vaccinated with most recent seasonal influenza vaccine
- having a signed informed consent form
Exclusion criteria
The exclusion criteria are:
- immune deficiencies
- haematological disorders
- bleeding disorders
- usage of anticoagulants, corticosteroids, NSAIDs and/or statins
- diabetes mellitus
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL22965.041.08 |