No registrations found.
ID
Source
Brief title
Health condition
microbiota and immune cells in IBD
Sponsors and support
Intervention
Outcome measures
Primary outcome
1. Intra-individual differences between inflamed and non-inflamed tissue in presence of microbial DNA in specific microenvironmental spaces; differences between IBD patients and controls;
2. Intra-individual differences in presence of microbial DNA in specific microenvironmental spaces of sigmoid mucosa before and 12 weeks after diverting ostomy;
3. Identify adaptive and innate immune cells producing cytokines involved in IBD.
Secondary outcome
1. Presence of microbial DNA in granuloma’s of CD patients;
2. Assessment of co-localisation between invading microbes and lamina propria macrophages dendritic cells using FISH probes;
3. Alterations between inflamed and non-inflamed tissue between patients and controls of phagosome maturation and autophagy, as well as apoptosis of mucosal macrophages and dendritic cells in IBD;
4. Assessment of signalling between innate lymfoid cell (ILC) subsets and lamina propria macrophages dendritic cells;
5. Assessment of ILC’s subpopulations in peripheral blood.
Background summary
Inflammatory bowel disease (IBD) consists of Crohn’s disease (CD) and ulcerative colitis (UC). The current prevailing hypothesis states that the pathogenesis involves an inappropriate and ongoing activation of the mucosal immune system driven by the intestinal microbiota in a genetically predisposed individual. However, it is not known which constituents of the microbiota and which components of the innate and adaptive immune response are involved. The human microbiota forms a complex ecosystem with its host and may comprise more than 1800 phylotypes.
The aim of our project is in-depth characterization of the microbiota components both adjacent to and within the mucosa in patients with IBD, by applying novel high-throughput analysis technology in a global description strategy approach. Furthermore, we aim to assess the interaction between invading microorganisms, local housekeeping cells and the adaptive and innate immune system in the intestine.
Study objective
We hypothesize that the perturbed interplay between certain constituents of the microbiota and the host is caused by invading organisms, which after crossing the epithelial barrier are capable of inciting and/or persisting an unbalanced innate immune response because clearing of microorganisms is impaired by dysfunction of the local housekeeping cells of the innate immunity.
Study design
Diverting ostomy group: Week 0, 12.
Intervention
N/A
Academic Medical Center Amsterdam<br>
Meibergdreef 9, C2-231
N.G.M. Rossen
Amsterdam 1105 AZ
The Netherlands
+31 (0)20 5662199
N.G.Rossen@amc.uva.nl
Academic Medical Center Amsterdam<br>
Meibergdreef 9, C2-231
N.G.M. Rossen
Amsterdam 1105 AZ
The Netherlands
+31 (0)20 5662199
N.G.Rossen@amc.uva.nl
Inclusion criteria
1. Patients ≥18 years or older;
2. Established Crohn’s disease, ulcerative colitis and CRC patients not known with IBD who undergo intestinal resection;
3. Patients with active left-sided ulcerative colitis or Crohn’s colitis who are scheduled for diverting ostomy.
Exclusion criteria
1. Ischemia of the bowel;
2. Positive stool cultures or parasite tests for common enteric pathogens;
3. Use of Antibiotics in preceding 4 weeks;
4. Use of probiotics in preceding 8 weeks;
5. Radiation therapy within 4 weeks before surgery;
6. Chemotherapy within 4 weeks before surgery.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| NTR-new | NL2768 |
| NTR-old | NTR2908 |
| Other | METC AMC : 10/338 |
| ISRCTN | ISRCTN wordt niet meer aangevraagd. |