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ID
Source
Brief title
Health condition
Digestion
Sponsors and support
Intervention
Outcome measures
Primary outcome
Adults: true metabolic availability of L-lysine in two milk powders compared with a reference crystalline amino acid mixture
Children: relative metabolic availability of L-lysine in two milk powders
Secondary outcome
NA
Background summary
Protein quality of a food reflects its ability to meet daily tissue amino acid requirements depending on the content, composition and metabolic availability of constituent amino acids. However, to ensure product quality, commercial products are subjected to varying degrees of processing, which leads to loss of bio-availability of proteins due to structural alterations of the amino acids. Therefore, in the present study, our aim is to determine and compare metabolic availability of L-lysine from two differently processed milk powders, among healthy adults (n=7) and children (n=12) using a simple, non-invasive and rapid technique called indicator amino acid oxidation method. The metabolic availability of L-lysine from the milk proteins will be estimated by comparing the indicator oxidation response to varying intakes of L-lysine from milk proteins with the response to varying intakes of synthetic L-lysine using slope ratio method. Similar concept will be applied to determine relative metabolic availability of one milk protein against the reference in the children study. Therefore, the present study will be instrumental in understanding the effect of processing on milk protein bio-availability.
Study objective
Different processing of milk products will have impact on metabolic availability of amino acid
Study design
Adults: 7 study days with one week wash-out in between study days
Children: 5 study days with one week wash-out in between study days
Intervention
Two different milk powders
Inclusion criteria
Adults:
Apparently healthy adult male volunteers
Normal Hb > 13g/l
Age: 20-40 years
Normal BMI between 18.5 to 25 kg/m2
Physical activity level <1.5 assess nu validated questionnaire
Subject to agree to avoid smoking and alcohol consumption for the entire study duration
Subject willing to participate and adhere to the study protocol for 7 consecutive weeks
Children:
Children 6-10 years of age, both sexes
Apparently healthy
BAZ between -2SD and 1 SD
Children and parents willing to participate and adhere to adaptation diets for 5 consecutive weeks
Exclusion criteria
Adults:
History of smoking or taking other leisure drugs in past 3 months and/or study duration
Consumption of alcohol in the previous 24 hr and/or in study duration
History of antibiotics in last month
On iron, protein, multivitamin, and/ or any other supplementation therapy in past 3 months
Unstable weight over last 3 months
Use of medications known to affect intestinal functions and protein metabolism
Participation in any nutritional intervention study in last 3 months
History of food allergy
History of acute illness. gastointestinal, respiratory tract symptoms of fever in the past 1 week
diagnosed with any acute or chronic medical conditions
Children:
Any acute or chronic illnesses
Menarche
Weight loss in last 1 month
Worm infestation as assessed by stool test
History of antibiotics in last month
On iron, protein, multivitamin, and/ or any other supplementation therapy in past 3 months
Use of medications known to affect intestinal functions and protein metabolism
History of food allergy
Design
Recruitment
IPD sharing statement
Followed up by the following (possibly more current) registration
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Other (possibly less up-to-date) registrations in this register
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In other registers
| Register | ID |
|---|---|
| NTR-new | NL7991 |
| Other | Mahidol University Central Institutional Reveiw Board : MU-CIRB 2019/109.1906 |